Bone Healing Properties of Autoclaved Autogenous Bone Grafts Incorporating Recombinant Human Bone Morphogenetic Protein-2 and Comparison of Two Delivery Systems in a Segmental Rabbit Radius Defect
최은주, 강상훈, 권현진, 조성원, 김형준,
소속 상세정보
최은주 ( Choi Eun-Joo ) - Yonsei University College of Dentistry Department of Oral and Maxillofacial Surgery
강상훈 ( Kang Sang-Hoon ) - National Health Insurance Corporation Ilsan Hospital Department of Oral and Maxillofacial Surgery
권현진 ( Kwon Hyun-Jin ) - Yonsei University College of Dentistry Department of Oral Biology
조성원 ( Cho Sung-Won ) - Yonsei University College of Dentistry Department of Oral Biology
김형준 ( Kim Hyung-Jun ) - Yonsei University College of Dentistry Department of Oral and Maxillofacial Surgery
KMID : 1036320140360030094
Abstract
Purpose: This study aims to validate the effect of autoclaved autogenous bone (AAB), incorporating Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2), on critical-sized, segmental radius defects in rabbits. Delivery systems using absorbable collagen sponge (ACS) and fibrin glue (FG) were also evaluated.
Methods: Radius defects were made in 12 New Zealand white rabbits. After autoclaving, the resected bone was reinserted and fixed. The animals were classified into three groups: only AAB reinserted (group 1, control), and AAB and ErhBMP-2 inserted using an ACS (group 2) or FG (group 3) as a carrier. Animals were sacrificed six or 12 weeks after surgery. Specimens were evaluated using radiology and histology.
Results: Micro-computed tomography images showed the best bony union in group 2 at six and 12 weeks after operation. Quantitative analysis showed all indices except trabecular thickness were the highest in group 2 and the lowest in group 1 at twelve weeks. Histologic results showed the greatest bony union between AAB and radial bone at twelve weeks, indicating the highest degree of engraftment.
Conclusion: ErhBMP-2 increases bony healing when applied on AAB graft sites. In addition, the ACS was reconfirmed as a useful delivery system for ErhBMP-2.
키워드
Bone morphogenetic protein 2; Autogenous autoclaved bone; Critical bone defect; Rabbit radius; BMP carrier
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